Search results for "Air pouch"

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Novel anti-inflammatory chalcone derivatives inhibit the induction of nitric oxide synthase and cyclooxygenase-2 in mouse peritoneal macrophages

1999

AbstractIn a previous work, we tested a series of chalcone derivatives as possible anti-inflammatory compounds. We now investigate the effects of three of those compounds, CH1, CH8 and CH12, on nitric oxide and prostanoid generation in mouse peritoneal macrophages stimulated with lipopolysaccharide and in the mouse air pouch injected with zymosan, where they showed a dose-dependent inhibition with inhibitory concentration 50% values in the μM range. This effect was not the consequence of a direct inhibitory action on enzyme activities. Our results demonstrated that chalcone derivatives inhibited de novo inducible nitric oxide synthase and cyclooxygenase-2 synthesis, being a novel therapeuti…

LipopolysaccharidesChalconeLipopolysaccharidemedicine.drug_classBiophysicsNitric Oxide Synthase Type IILipopolysaccharidePharmacologyBiochemistryDinoprostoneAnti-inflammatoryNitric oxideMicechemistry.chemical_compoundChalconeStructural BiologyGeneticsmedicineAnimalsCyclooxygenase-2Mouse air pouchInducible nitric oxide synthaseMolecular BiologyNitritesDose-Response Relationship DrugbiologyAnti-Inflammatory Agents Non-SteroidalZymosanZymosanProstanoidMouse peritoneal macrophageCell BiologyIsoenzymesNitric oxide synthasechemistryBiochemistryCyclooxygenase 2Prostaglandin-Endoperoxide SynthasesEnzyme InductionMacrophages Peritonealbiology.proteinFemaleCyclooxygenaseNitric Oxide SynthaseFEBS Letters
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Dysidotronic acid, a new sesquiterpenoid, inhibits cytokine production and the expression of nitric oxide synthase.

2001

In a previous study, we reported a new bioactive sesquiterpenoid, named dysidotronic acid, to be a potent, selective human synovial phospholipase A(2) inhibitor. Dysidotronic acid is a novel, non-complex manoalide analogue lacking the pyranofuranone ring. We now investigate the effect of this compound on cytokine, nitric oxide and prostanoid generation on the mouse macrophage cell line RAW 264.7, where it showed a dose-dependent inhibition with inhibitory concentration 50% values in the micromolar range. This effect was also confirmed in the mouse air pouch injected with zymosan. Dysidotronic acid inhibited the production of tumor necrosis factor alpha and interleukin-1 beta as well as the …

Nitric oxide (NO)MouseLeukotriene B4NeutrophilsRAW 264.7Dysidotronic acidNitric Oxide Synthase Type IIDinoprostonePhospholipases ANitric oxideCell Linechemistry.chemical_compoundManoalideMicemedicineAnimalsHumansProstaglandin E2Enzyme InhibitorsCytokineNitritesPharmacologybiologyTumor Necrosis Factor-alphaMacrophagesZymosanZymosanMembrane ProteinsNitric oxide synthaseIsoenzymesAir pouchchemistryBiochemistryEnzyme inhibitorCyclooxygenase 2Prostaglandin-Endoperoxide Synthasesbiology.proteinCytokinesArachidonic acidDiterpenesNitric Oxide SynthaseSesquiterpenesmedicine.drugEuropean journal of pharmacology
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